Composite

Part:BBa_K2477010:Design

Designed by: Paula Kleyman, Thea Ornstein, Yuzhu Shi, Joyce Song   Group: iGEM17_UMaryland   (2017-10-26)


TLP Secretion System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2180
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2119
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 181
    Illegal AgeI site found at 284
    Illegal AgeI site found at 326
    Illegal AgeI site found at 1954
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 468
    Illegal SapI site found at 1936


Design Notes

We had to get rid of the stop codons inbetween the two parts following 3A assembly.


Source

TLP is from Oryza Sativa. The other parts are from the iGEM kit.

References